Browsing by Author "Ariyadasa, Thilini U."

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    Novel strategy for microalgae cell disruption and wet lipid extraction by employing electro-Fenton process with sacrificial steel anode
    (Elsevier, 2022) Sandani, Wanniarachchige Paramitha; Premaratne, Malith; Ariyadasa, Thilini U.; Premachandra, Jagath Kumara
    Electro-Fenton process (EFP) was studied as a potential cell disruption technique for recovery of lipids from wet biomass of the microalga Chlorella homosphaera. A novel approach of electrochemical dissolution of a sacrificial steel anode was used to provide Fe2+ required to initiate EFP and microalgae cell disruption. Response surface methodology (RSM) was employed to optimize the process parameters and maximize the lipid yield of EFP. The RSM model (R2 = 90.66%, Adj.R2 = 87.71%) showed that a maximum lipid yield of 18.29% could be obtained at 40 min reaction time and 4.38 g/L biomass concentration. Experimental validation resulted in a lipid yield of 19.99 ± 1.33%, which was significantly higher than wet lipid extraction without cell disruption. However, the lipid yield of EFP should be further improved to achieve comparable results to mechanical cell disruption methods. Nonetheless, biodiesel synthesized from lipids obtained via EFP conformed to the ASTM D6751-12 standard.
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    A novel two-stage process for the effective treatment of swine wastewater using Chlorella sorokiniana AK-1 based algal-bacterial consortium under semi-continuous operation
    (Elsevier, 2022) Chen, Chun-Yen; Kuan, Shu-Ping; Nagarajan, Dillirani; Chen, Jih-Heng; Ariyadasa, Thilini U.; Chang, Jo-Shu
    This study aimed at developing an eco-friendly and effective treatment for swine wastewater (SWW) using a designer microalgae-bacteria consortium. A functional algal bacterial consortium was developed with SWWderived bacteria and Chlorella sorokiniana AK-1. Light intensity (300 μmol/m2/s) and inoculum size (0.15 and 0.2 g/L for microalgae and bacteria) were optimized. Semi-batch operation treating 50 % SWW resulted in a COD, BOD, TN, and TP removal efficiency of 81.1 ± 0.9 %, 97.0 ± 0.7 %, 90.6 ± 1.6 % and 91.3 ± 1.1 %, respectively. A novel two-stage process with an initial bacterial start-up stage followed by microalgal inoculation was applied for attaining stable organic carbon removal, in addition to satisfactory TN and TP removal. Full strength SWW was treated with this strategy with COD, BOD, TN, and TP removal efficiencies of 72.1 %, 94.9 %,