Development of new kinetic model for meshophilic biohydrogen production in Trickle bed reactor using clostrodium butyricum CWBI 1009

Abstract

The aim of present study is to develop a new kinetic model for bio hydrogen production in biofilm by Clostridium butyricum CWBI1009 grown in Trickle Bed reactor running on sequence batch mode. Bio hydrogen production experiments had already been conducted using Trickle Bed Sequence Batch Reactor (TBSBR) using Clostridium butyricum CWBI1009. Measured parameters were hydrogen composition, hydrogen production rate and soluble metabolites. Controlled parameters were pH, recirculation flow rale and temperature. This reactor was operated for 62 days. Modified Anaerobic Digestion Model No.J. /(ADM1) is used to model initial phase of TBSBR. The reactor configuration, input substrate and synthetic medium were set as dictated by original ADM1. Tvvo sets of parameters were investigated in this study. In the first phase, parameters given by ADM1 were applied. In the second phase, parameters were adjusted in the range given by ADM1 until measured output and model output are in close agreement. Following parameter estimation, pH value was in close agreement with reactor operational pH value, gas flow rates and hydrogen percentage follows same Hj|;em as experimental values within 1-2% variation. However, modelled concentrations of volatile tatty acids were not well fitted with experimental data.