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Rapid degradation of FOG discharged from food industry wastewater by lipolytic fungi as a bioaugmentation application

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dc.contributor.advisor Ratnayake, N
dc.contributor.advisor Manathunga, J
dc.contributor.advisor Nanayakara, CM
dc.contributor.author Witharana, WAU
dc.date.accessioned 2018-12-05T21:14:08Z
dc.date.available 2018-12-05T21:14:08Z
dc.identifier.uri http://dl.lib.mrt.ac.lk/handle/123/13714
dc.description.abstract Rapid Degradation of FOG Discharged from Food Industry Wastewater by Lipolytic Fungi as a Bioaugmentation Application Fats, oils, and grease (FOG) congregate in grease trap devices as a result of culinary activities in the foodservice industry. FOG is considered to be slowly biodegradable particulate (sbpCOD) organic matter and may require enzymatic or hydrolytic conversion to form readily biodegradable soluble organic matter (rbsCOD). The existing treatment methods are claimed on water-based hydrolysis of FOG to form long chain fatty acids. The long chain fatty acids discharged into wastewater treatment systems create functional difficulties, especially the inhibitory effect caused by the accumulation of such fatty acids. In order to overcome the issues associated with water-based treatment systems, FOG was extracted from the waste and solid-state degradation was performed by lipolytic fungi in a traytype reactor as a novel approach of bioaugmentation. In the reactor, each 10 mg/g dry weight of FOG (substrate) was mixed with 1% w/v of coir fiber for proper aeration. Then the reactor was inoculated with 1 mL of spore suspension (1 × 107 spores/mL) of lipolytic fungi. The isolated lipolytic fungi were Aspergillus niger, Geotrichum candidum, Aspergillus fumigates, Fusarium proliferatum and Penicillium citrinum. The optimum conditions to degrade grease trap waste by solid-state degradation: initial moisture content of FOG should be 25 – 35% of weight; temperature 30°C; pH should be between 6–7; the reactor moisture condition for continuation of degradation process should be maintained around 65%. The higher degradation efficiencies (>80%) were recorded by these fungi isolates. As a practical application of the developed methodology, solid-state degradation was performed with raw grease trap waste (without extraction of FOG) in room temperature without adjusting the pH. The recorded pH for grease trap waste varied between 4.5–6.5 and most abundant fatty acids present in grease trap waste were palmitic acid 49.5% (w/w) and oleic acid 33% (w/w). Within 72 h of post-incubation, degradation efficiency of about 50% was recorded by fungal isolates. The degraded residue can be used as an inoculum for the degradation of the second set of grease trap waste. Therefore, once the degradation cycle is started, continuous inoculation for the rest of the degradation process would not be needed. The feasibility of using the developed protocol for FOG degradation was tested with a laboratory-scale tray type reactor, and it was operated successfully. en_US
dc.language.iso en en_US
dc.subject Fatty acid methyl esters en_US
dc.subject grease trap waste en_US
dc.subject lipase, long-chain fatty acid en_US
dc.subject solid state degradation en_US
dc.title Rapid degradation of FOG discharged from food industry wastewater by lipolytic fungi as a bioaugmentation application en_US
dc.type Thesis-Abstract en_US
dc.identifier.faculty Engineering en_US
dc.identifier.degree Doctor of Philosophy (PhD) en_US
dc.identifier.department Department of Civil Engineering en_US
dc.date.accept 2018-07
dc.identifier.accno TH3670 en_US


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